한국콩연구회

분자표지를 이용한 SMV 및 불마름병 저항성 유전자 집적 콩 품종 개발 및 보급

Development and distribution to growers of soybean cultivars containing resistance genes to SMV and bacterial pustule diseases pyramided by marker-assisted selection

주관연구기관 : 한국생명공학연구원

발행년월 : 2011-02

주관부처 : 농촌진흥청

원문

http://www.ndsl.kr/ndsl/commons/util/ndslOriginalView.do?dbt=TRKO&cn=TRKO201100016012&rn=&url=&pageCode=PG18

초록

Soybean is the world's leading oil and protein source for humans. Also, soybean is the second-largest food crop in cash sales after the rice in Korea. Soybean mosaic virus (SMV) and bacterial leaf pustule (BP), caused by Xanthomonas axonopodis pv. glycines, are one of the major viral and bacterial diseases in soybean fields of Korea. However, it is impossible to control the viral and bacterial diseases with successive treatment of fungicides, pesticides and insecticides during the growing season. Growing resistance

cultivars to SMV and BP is the most cost-effective and environment-friendly ways. To develop multiple resistance lines with all three SMV resistance loci (RsvLPI96983, Rsv3;L29 and RsT4;V94-5152) and BP resistance locus (rxp), crosses were made between Rsv-pyramided SMV resistance lines and BP resistance lines, which were developed in previous project. In this study, we conducted marker-assisted selection (MAS) with closely linked flanking markers of SSR or SCAR to each resistance loci. Rapid generation advancement by taking 2 to 3 generations per year and MAS at each generation were conducted to select individuals homozygous for the each resistance loci. As a result of MAS breeding, we could select a total of 27 four-gene-pyramided F6 lines (RsVlRsVlRsv3Rsv3RsT4RsT4rxprxp) from crosses among the 'Taekwang' genetic background lines. In addition, more than 500 lines which were homozygous at 2 or 3 resistance loci, were successfully selected in this experiment. Resistance lines with good agricultural performances were selected from field evaluation including bioassay for SMV and BP in 2010.

연구개발의 내용 및 범위

1. SMV 및 불마름병 복합저항성 콩 계통 개발 (제1협동과제)

+ 복합 병저항성(SMV 및 불마름병) 콩 계통 개발 : two-genes ~ four-genes pyramided line 구축

2. SMV 저항성 유전자 연관 마커 개발 및 유전자 분리 (제1세부연구과제)

+ 장려품종들에 다형성이 높고 실용성이 있는 SMV 저항성 유전자 근접 마커 개발

+ 광범히 저항성이며 새로운 유형의 유전자로 예측되는 SMV 저항성 유전자 Rsv4 의 상세 유전지도

작성

+ Rsv4 유전자의 positional cloning 및 모텔식물 형질 전환

3. 콩 불마름병 저항성 유전자 분리 및 연관 마커 개발 (제2협동연구과제)

+ 불마름병 저항성 유전자에 대한 near isogenic line (NIL) 육성

+ GS-FLX를 이용한 콩 불마름병 N표로부터 불마름병 저항성 유전자 동정

+ 불마름병 저항성 유전자에 대한 연관마커 개발 및 상세 유전자 지도 작성

+ 불마름병 저항성 유전자의 모델식물에서의 형질 전환

4. SMV 및 불마름병 복합 저항성 콩 계통 지역적웅시험 및 농가보급 (제3협동연구과제)

+ 복합 병저항성 콩 품종개발을 위한 지역적응시험 및 개발 품종의 조기 농가 보급 : '08 -'10